Journal: Journal of the American Geriatrics Society

Article Title: Hiring and Screening Practices of Agencies Supplying Paid Caregivers to Older Adults

doi: 10.1111/j.1532-5415.2012.04047.x

Figure Lengend Snippet: Paid Caregiver Agency Selection

Article Snippet: Agencies also reported performing a National Caregiver Background Check (n=1), National Scantron Test for Inappropriate Behaviors (n=1), Assessment of Certification of Christian Morality (n=1), and Quality Seal of Assurance Online Program Completion (n=1); we were unable to confirm the existence of any of these.

Techniques: Selection

Journal: Journal of the American Geriatrics Society

Article Title: Hiring and Screening Practices of Agencies Supplying Paid Caregivers to Older Adults

doi: 10.1111/j.1532-5415.2012.04047.x

Figure Lengend Snippet: Characteristics of Paid Caregiver Agencies - n (%) (n=180)

Article Snippet: Agencies also reported performing a National Caregiver Background Check (n=1), National Scantron Test for Inappropriate Behaviors (n=1), Assessment of Certification of Christian Morality (n=1), and Quality Seal of Assurance Online Program Completion (n=1); we were unable to confirm the existence of any of these.

Techniques:

Journal: Journal of the American Geriatrics Society

Article Title: Hiring and Screening Practices of Agencies Supplying Paid Caregivers to Older Adults

doi: 10.1111/j.1532-5415.2012.04047.x

Figure Lengend Snippet: Hiring Requirements of Paid Caregiver Agencies - n (%) (n=180)

Article Snippet: Agencies also reported performing a National Caregiver Background Check (n=1), National Scantron Test for Inappropriate Behaviors (n=1), Assessment of Certification of Christian Morality (n=1), and Quality Seal of Assurance Online Program Completion (n=1); we were unable to confirm the existence of any of these.

Techniques: Drug discovery

Journal: Journal of the American Geriatrics Society

Article Title: Hiring and Screening Practices of Agencies Supplying Paid Caregivers to Older Adults

doi: 10.1111/j.1532-5415.2012.04047.x

Figure Lengend Snippet: 10 Questions to ask agencies prior to hiring a paid caregiver

Article Snippet: Agencies also reported performing a National Caregiver Background Check (n=1), National Scantron Test for Inappropriate Behaviors (n=1), Assessment of Certification of Christian Morality (n=1), and Quality Seal of Assurance Online Program Completion (n=1); we were unable to confirm the existence of any of these.

Techniques:

Journal: ACS Chemical Neuroscience

Article Title: Neurotropic activity and safety of methylene-cycloalkylacetate (MCA) derivative 3-(3-allyl-2-methylenecyclohexyl) propanoic acid

doi: 10.1021/acschemneuro.0c00255

Figure Lengend Snippet: HU- MCA-13 induced neurotropic effect in different neuronal cultures. Neurotropic effect was measured after treatment with 5 μM of HU-MCA-13 of rat PC12 dopaminergic neurons (7 days, top row), mice dorsal root ganglion (DRG) explants (5 days, middle row) and rat spinal cord sensory neurons (14 days, bottom row). The negative control cultures were treated with 0.1% DMSO and the positive control cultures were treated with 50 ng/mL mouse β-nerve growth factor (NGF) for the same periods of time.

Article Snippet: Tissue culture grade mouse β-NGF, was purchased from Alomone Laboratories (Jerusalem, Israel).

Techniques: Negative Control, Positive Control

Journal: Frontiers in Microbiology

Article Title: Rift Valley Fever Virus Is Lethal in Different Inbred Mouse Strains Independent of Sex

doi: 10.3389/fmicb.2020.01962

Figure Lengend Snippet: Nearly all “survivor” mice in the 0.2 TCID 50 challenge group were not infected with Rift Valley fever virus (RVFV). (A) Survival curve of five inbred mouse strains infected via footpad injection with wild-type RVFV at a dose of 0.2 TCID 50 shows “survivor” mice across all five strains. Each line represents the percent survival after infection of five female and five male mice. (B) Enzyme-linked immunosorbent assay (ELISA) and qRT-PCR analysis of “survivor” mice as well as early and late-death 0.2 TCID 50 -challenged mice as controls. Endpoint RVFV-specific ELISA titer in the serum of mice that survived to day 28 post-infection. Viral load per milligram of tissue in the liver, spleen, brain, and testes of mice that survived to day 28 post-infection was measured by qRT-PCR. The limits of detection for these assays are indicated by dashed lines (ELISA limits of detection due to limited dilutions: lower limit of 100 and upper limit of 12,800; qRT-PCR limit of detection: 77.5 RNA copies/mg).

Article Snippet: Titers were determined through visualization by indirect fluorescent antibody assay (IFA) using a 1:500 dilution of a custom RVFV anti-N polyclonal #5584 (Genscript) as primary antibody and a 1:500 dilution of anti-rabbit 488 (Invitrogen) as secondary antibody.

Techniques: Infection, Virus, Injection, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Frontiers in Microbiology

Article Title: Rift Valley Fever Virus Is Lethal in Different Inbred Mouse Strains Independent of Sex

doi: 10.3389/fmicb.2020.01962

Figure Lengend Snippet: Lethality in five inbred mouse strains following RVFV infection. (A) Survival curves of five inbred mouse strains infected via footpad injection with wild-type RVFV at doses of 0.2 or 2 TCID 50 show dose-dependent differences in time to death but not survival. Each line represents the percent survival after infection of female and male mice at a given challenge dose. Confirmed uninfected mice from the 0.2 TCID 50 dose are excluded from the graphs. (B) Percent survival of all five inbred mouse strains when infected with 2 TCID 50 RVFV. Each line represents the percent survival after infection of five female and five male mice for a given strain. (C) Percent change in mouse daily weight from baseline in all five inbred strains after infection with 2 TCID 50 RVFV. Weight loss curves represent five female and five male mice for a given strain. Survival statistics were calculated using a log rank (Mantel-Cox) test and p are marked on all graphs. The mixed-effects model with the Geisser-Greenhouse correction were used for weight loss comparisons between mouse strains over time.

Article Snippet: Titers were determined through visualization by indirect fluorescent antibody assay (IFA) using a 1:500 dilution of a custom RVFV anti-N polyclonal #5584 (Genscript) as primary antibody and a 1:500 dilution of anti-rabbit 488 (Invitrogen) as secondary antibody.

Techniques: Infection, Injection

Journal: Frontiers in Microbiology

Article Title: Rift Valley Fever Virus Is Lethal in Different Inbred Mouse Strains Independent of Sex

doi: 10.3389/fmicb.2020.01962

Figure Lengend Snippet: Sex does not impact survival and has only a modest effect on time to death and weight loss in five inbred mouse strains following RVFV infection. (A) Survival curves of five inbred mouse strains infected via footpad injection with 2 TCID 50 RVFV show no difference between female and male mice. Each line represents the percent survival after infection of five mice of each sex. (B) Percent change in mouse daily weight from baseline after infection with 2 TCID 50 RVFV. Each weight loss line represents five mice. Survival statistics were calculated using a log rank (Mantel-Cox) test and p are marked on all graphs. The mixed-effects model with the Geisser-Greenhouse correction and Bonferroni’s multiple comparisons test were used for weight loss comparisons between sexes and over time for each mouse strain. An asterisk at a particular time point indicates significance ( p ≤ 0.05) in post hoc analysis.

Article Snippet: Titers were determined through visualization by indirect fluorescent antibody assay (IFA) using a 1:500 dilution of a custom RVFV anti-N polyclonal #5584 (Genscript) as primary antibody and a 1:500 dilution of anti-rabbit 488 (Invitrogen) as secondary antibody.

Techniques: Infection, Injection

Journal: Frontiers in Microbiology

Article Title: Rift Valley Fever Virus Is Lethal in Different Inbred Mouse Strains Independent of Sex

doi: 10.3389/fmicb.2020.01962

Figure Lengend Snippet: Quantification of viral RNA levels in key tissues of five inbred mouse strains shows no statistically significant dose, sex, or strain differences. (A) Viral load per milligram of tissue in the liver, spleen, and brain of mice at the point of death or euthanasia after RVFV infection at doses of 0.2, 2, 20, 200, and 2,000 TCID 50 . Confirmed uninfected mice from the 0.2 TCID 50 dose are excluded from the graph. (B) Viral load per milligram of tissue in the liver, spleen, brain, and testes of mice that succumbed to infection with 2 TCID 50 RVFV was measured by qRT-PCR. A one-way ANOVA was used to compare viral RNA loads within each tissue for each strain across doses. For NZO/HILtJ mice an unpaired t -test was used since there were only two challenge doses. A two-way ANOVA was used to compare viral RNA loads within each tissue across strains and sex. A one-way ANOVA was used to compare viral RNA loads within the testes across strains. The limit of detection of these assays (77.5 RNA copies/mg) is indicated by a dashed line.

Article Snippet: Titers were determined through visualization by indirect fluorescent antibody assay (IFA) using a 1:500 dilution of a custom RVFV anti-N polyclonal #5584 (Genscript) as primary antibody and a 1:500 dilution of anti-rabbit 488 (Invitrogen) as secondary antibody.

Techniques: Infection, Quantitative RT-PCR